Organizational culture is the environment in the workplace formulated through the interaction of employees’ at work which can be learned in the social environment and can encourage the innovative behavior among members of the organization. This study was conducted on the effects of organization culture on employees’ motivation from 2019-2020 G.C. and it’s aim was to assess the effect of organizational culture on employees’ motivation in Burie Cluster Branches of ACSI. Organizational culture dimensions namely involvement, consistency, adaptability and mission were considered as independent variables whereas the dependent variable was employees’ motivation. This study is done in a quantitative research approach, a cross sectional research methods, and explanatory, deductive and theory testing research type. A 5-point Likert scale format questionnaire was used to collect primary data. A total of 200 questionnaires were distributed to the professional employees’ of the organization and 186 (93%) questionnaires were returned. The questionnaire had a Cronbach alpha coefficient of between 0.7 up to 0.9 suggesting that the instrument was reliable. Data analysis was done using Statistical Package for Social Sciences (SPSS) version 20 and used descriptive and inferential statistics to analyze the data. The correlation between the independent variable organizational culture traits and the dependent variable employees’ motivation indicates that positive and significant. Then also, linearity, normality and multi co linearity regression tests were applied to determine the contribution of each independent variable in employees’. The results show that there is strong and moderate statistical positive correlation between dependent and independent variables. Furthermore, multiple regression analysis is used to measure the statistical effect of each individual independent variable on the dependent variable. The test result indicated that the alternative hypothesis of involvement, consistency adaptability, and mission are supported by the multiple regressions because the p<0.05. Finally, the researcher recommended that the company should continue and enhance those stated culture traits specially involving all stakeholders in decision making, sharing the organization mission and vision to all level employees’, and create policies that encourage employees’ and managers to adapt new changes inside and outside the organization and to update themselves.