Women constitute half of humankind and 40% of the global workforce. Many gender issues which are very important to well being of millions of women and girls around the world got public attention. The participation of women in agricultural work, especially in private farm enterprises has been growing from time to time. However, the study about the challenges and opportunities of women labor workers in the private farm enterprise is not well studied. From this gap, the study attempted to explore challenges and opportunities of women labor workers by taking Ayehu farm enterprise as a case. To meet the objective of the study, qualitative research method with case study research design was employed. In this study, eight semi structured interviewees, five key informant interviewees and twelve focus group discussants were participated. In this study, non probability purposive sampling and availability sampling was employed to select the participants of the study. This study was employed both primary and secondary data sources. Semi structured interview, key informant interview and focus group discussion were used as a tools for collecting primary data and labor agreement document review for secondary data. The data collected were analyzed through thematic analysis methods. The findings of the study revealed that women labor workers of the farm faced different challenges concerning administrative, economic and social aspects. In terms of administration (bureaucratic despot, lack of follow up lack of facilities and job insecurity); economic (lower payment and labor exploitation); social challenges (psychological trauma, gender based violence, and low respect from community). On the contrary, women labor workers of the farm enterprise had some opportunities as administrative (transport services, upgrading/promotion, sick leave, maternity leave and other miscellaneous permission); economic (job opportunities, piece rate payment and life insurances); social (health service, working in a familiar environment, cereal food and housing provision). The study has concluded that women labor workers of the farm have faced several challenges with some opportunities they reap. Based on