Fruit flies (Diptera: Tephritidae) are one of the most diverse group of insects, pose a major threat to horticulture. A study was conducted to characterize the species composition of fruit flies attacking fruits and damage caused in selected areas of Eastern Amhara. Sample fruit flies were collected using two methods; rearing from infested host fruits and trapping the adults with attractants. For damage level determination samples of ripe fruit of Mango, Guava and sweet orange were collected for four consecutive weeks. Three fruit fly species were recovered from the fruits collected Bacterocera dorsalis, Ceratitis cosyra and Ceratitis capitata; four fruit fly species were trapped Bacterocera dorsalis, Ceratitis cosyra, Ceratitis capitata and Ceratitis fasciventris. Bacterocera dorsalis was the most abundance species accounted for 97.9 and 78.89% of the total trapped and emerged adults respectively; and Ceratitis fasciventris was observed only from the trap with the lowest proportion of trapped adults (0.03%), whereas Ceratitis cosyra had the lowest proportion of emerged adults (3.92%). The analysis of variance table for the trapped Bacterocera dorsalis and Ceratitis capitata per trap per week showed a significant difference among trapping locations, attractants and weeks, The highest number of B. dorsalis per trap per week were trapped at Kalu (107.28) followed by Kobo (64.17) and Habru (16.46). Ceratitis capitata was higher at Habru (1.20) followed by Kalu (0.75) and Kobo (0.42); significantly higher adults trapped in male lure traps (97.03%) compared to food based attractants (2.97%). The means of damaged fruits (incidence percentage) recorded 33.36±3.04, 33.96±2.74, 24.02±5.22 and 25 for Mango, 73.56±3.91, 78.32±4.26, 80.24±2.86 and 80.60 for Guava and 1.04±1.04, 14.07±3.07, 4.36±2.33 and 2.94 for Sweet orange at Kobo, Habru, Kalu and Shewarobit respectively. The overall significantly highest percentage of fruit damage was observed on guava (79.76), followed by mango (28.26) and 3.8% on sweet orange. Infestation rates of 53.12, 15.7 and 2.67 kg-1; 5.72, 3.82 and 0.67 per fruit adult fruit flies on Guava, Mango and Sweet orange respectively. Suppression of fruit fly population could focus on Guava and Mango with respect to fruit fly species B. dorsalis and C. capitata in the area. Further studies over an extended period of time and fruit producing areas is necessary. Key words: B. dorsalis, C. capitata, Guava, Sweet orange, male lures, Mango