The speed of opening roller and rotor of a rotor spinning machine are among various parameters which can influence the quality of spun yarn. Different Opening roller and rotor speed have been used for the production of 20 Ne 100% cotton carded yarn at Bahir dar Textile Share Company to caryout this research work. Strength, elongation, twist amount, and evenness have been investigated for all the samples. Based on the result the optimum opening roller speed(8800rpm) and rotor speed (80000 rpm) for yarn tenacity, twist and unevenness, Where as for yarn elongation the opening roller speed (8800rpm) and rotor speed (75000rpm) values are suitable for 20/1 Ne. When the opening roller speed increase up to 8800rpm and rotor speed 80000rpm to improve yarn properties such as (tenacity, elongation, twist and imperfection