Loops are the main structural elements of knitting their shape and size influences the appearance and properties of knitted products even though other knitting and yarn parameters remain constant. In knitting industries, many derivatives of single jersey construction can be developed by combining different stitches for different reasons mainly; for design purpose, to give stability and to improve other physical properties. However, each stitch has its own effect on dimensional as well as comfort properties of a given knitted fabrics. This paper is focused on the effect of different stitch combinations mainly; knit, tuck and miss stitch on dimensional and comfort properties of single jersey derivative fabrics. Samples were knitted from 100% cotton yarn on circular knitting machines and the various dimensional and comfort properties were measured using relevant standards. From this investigation, it was found that the presence of tuck and float stitch in a given structure have significant effect on fabric drape ability, stretch and recovery, shrinkage, thickness, areal density, air permeability and low stress mechanical properties.