The major purpose of this research was challenges and opportunities of grade 11thand 12th female student’s participations in physical education practical class in the case of Guhala secondary and preparatory school in Amhara Region, Central Gondar Zone, East Belessa Woreda. The total population of the study were 241 (100%) and 85 (35.2%) sample were taken out of which 79(33.6%) female students,3 school administrators and 3 physical education teacher were selected for the study. Compressive and random sampling method was used in the study. Data collected from questioner, interview, and observation the participant were analyzed using tables and interpreted by both qualitative and quantitative analysis method. Some of the major findings include the majority of female students in the sample school have less interest to participate in physical education practicalclass,interest, confidence, motivation; family, school society, peers, and other communities; physical impairment, menstruation cycle; past experience; school facility and equipment;environmental weather conditionspecially hot temperature; roles of concerned body, did not improve the opportunities are major factors that hinder the participation of female students during physical education practical class. Hence, it is recommended that depending on the result of findings, concerned bodies such as parents, physical education teachers, Zone and Woreda educational bureau, etc, Should create awareness for female students about the benefits of physical education subject. Physical education teachers should apply student centers teaching approach in order to motivate female students.