The aim of this study was to investigate effect of ten weeks lower limbs strength training on 60m sprinting performance in Arbaminch University sport science department first year male students’. Non probability (purposive) sampling technique was used to select 20 male students aged 19 to 24 years from the total 62 first year students in Arbaminch University sport science department. The participants (these are the experimental groups) under this study took part in a followed exercise program performed in different lower limbs strength training for consecutive ten weeks. The activity was given from February to half of April / 2018, with the frequency of 4 days per week for 2 and 1/2 months and the duration was 40 minutes per session. The physical fitness variables selected for the study were: top speed and acceleration. Data’s were analyzed by using SPSS version 23 paired samples t-test with pair wise comparison of means at 95% confidence interval by using pre and post test. The results indicated that there were significantly improvements in performance on selected physical Fitness variables due to the effects of lower limbs strength training with p confirmed <0.05. That lower limbs strength training was significant to improve the speed and acceleration variables. The mean difference (MD) between pre and post tests for: the speed of students was improved by 0.6335m/s and for acceleration were improved 0.1235m/s2. The main finding of the study was students who were exposed to lower limbs strength training have discovered positive outcomes towards the speed, acceleration and in general sprinting performance of the students. Coaches, sport science teachers and athletes should include lower limbs strength training in their training plan in order to improve the sprinters speed and maximum acceleration.