he need for bioethonol is tncreasing worldwide duy' to day due to the need for renewable resources and ease of production from cheap raw materials. The main objective of ihis research L5 lo/111dy the possibility of producing bioetharo! from mJQ<:(uia am/ mango seeds through the separate hydrolysis and [ermentation (S!{F) process using the Saccharomyces cerevisiae yeast. Ethanol is a clear, colourless; jlammabie. oxygenated hydrocarbon with the chemical formula C~IlsON. Ethanol has been mad« .. since ancient rimes byjermemin:; sugars. Ethano! .fimnenwrion from hydro(v:ed mango or avocado seed WCJ.J analyzed in the present research and optimized by 11si11g diffemmfacwrs. the effects of hydrolysis time and acid concentratton on the amount of w10/ sugar concentration and 1'Jla1 of fermenuuion time and pll on the ethanol yield were studied . Maximum tow/ .wgar content from :\fanga seeds 32%(1v!W) wCLt recorded after 4 days hyd1·oiysis using 2% H:if>O, and that of Avocado seeds 31. 7%(w!wj was recorded after 3 days using; the same acid concenmqton. At optimum pH (5.5), the maximum ethanol yield from mango seed: was 6. 05% . ' • • II ~ (w'v) aftb 3 days Jimnenlation while Ihm of A"ocado seeds was 5. 11% (••Iv) after 5 days of fernle11tation .