Back ground: Tuberculosis (TB) remains a major global health problem. The emerging epidemics of multi- and extensively drug-resistant (M/XDR) TB further imperil patients and public health. Although TB infection control guidelines are available for resource-limited settings, their implementation is believed to be inadequate. Studies conducted on risk of tuberculosis infection and disease associated with work in health care settings showed that the median prevalence of latent TB infection was 63% (range 33-79% across studies) Objective: To assess Tuberculosis Infection Control Practice and associated factors among Health Workers in Westgojjam zone, Northwest Ethiopia. Methods: Institution based quantitative cross sectional study triangulated with qualitative observation will be used for this study. Six hundred thirty four health workers will be selected by multistage probability sampling method and interviewed using structured questionnaire to collect data. Each selected health center will be audited using key informant interview and observational checklists. Data will be entered using Epi Info version 3.5.3 and analyzed by SPSS version 20. Odds ratio with 95% confidence interval will be used to identify the associated factors with the outcome variable. Result: Results will be displayed with tables and figures Period: June-September 2014 Total budget: The total cost of the research project 22026 ETB