An ethnobotanical study on medicinal plants was conducted in Gozamin district, Amhara National Regional State, North West Ethiopia. The overall objective of this study was to assess the status of traditional medicinal plants and to document the associated knowledge of the local communities of the district. Eight villages were selected randomely among the the total 26 villages. In all sample villages 80 informants were selected and interviewed two times. Key informants were selected by purposive random sampling whereas the other informants were selected randomly from the local people of the study area. The ethnobotanical data were gathered through interview and questionnaire. A total of 92 medicinal plants belonging to 83 genera and 50 families were recorded and used to treat both human and livestock ailments. Of these, 82.6% species are used to treat only human ailments, 4.35% species are used for livestock ailments only and 13.04% species are reported for both human and livestock ailments. These medicinal plants are used to treat about 47 types of human ailments and 11types of animal ailments. Of the total documented plant species, the largest diversity of species recorded belongs to four families including the Solanaceae (8.69%), Asteraceae (6.52%), Euphorbiaceae and Fabaceae (5.43%), and Herbs were the major growth form (38.04%). The study showed that the most frequently used plant parts for threating different diseases are leaves (46.6%) followed by roots (25.24%) and seeds (11.65%). Crushing and mixing was the major (43.3%) way of traditional medicinal plants preparation and the most common route of administration was oral (48.54%) while vaginal was the least (0.97%). The main threats to medicinal plants in the study area were new agricultural lands expansion (33.75%) and efferts to conserve traditional medicine plants is negligible. The current study showed that, there is little practice of keeping medicinal plants from damage. It is therefore, recommended that create a conducieve environment for traditional medicine practitioners and train them to share their secretive knowledge to the next generation and People need to be encouraged to cultivate and conserve medicinal plants in their home garden through training or education. Key words: Ethnobotany, Medicinal plants, conservation, Gozamin district