This study aimed to identify the distribution and diversity of benthic macroinvertebrates at Infranze River, Amhara region, Ethiopia. Benthic macro invertebrates were collected between 10-18November 2020 from three stations located at upstream, midstream and downstream of the river. Benthic macro invertebrates were collected by using Kik net with 500 micron mesh size combined with a rectangular quadrate with the size of 30 cm x 30 cm. Benthic macro invertebrates and physicochemical parameters of water were recorded from 39 sampling sites along Infranz River that represent the different geomorphologic zones such as mountainous (n=11), transitional (n=16), and floodplain zone (n=12). A total of 867 macro invertebrates from 20 orders and 51 families were collected. The results indicate that the study area was primarily dominated by the order Odonata, which comprises predatory flying insects, including dragonflies and damselflies, with a count of 314 individuals. However, Hemeptera was the most diverse with seven families as compared to odonata and epemeroptera. The diversity of benthic macro invertebrates were abundant at the transitional zone compared to the other two zones. This was due to higher anthropogenic disturbances and less favorable environmental conditions for macro invertebrate populations. The correlation between benthic substrates was positive and significant for sand, mud, gravel, detritus and silt. The majority of individuals (202) were discovered in gravel, representing 32 families