n this study three Iron(III) complexes with 1,10-Phenanthroline, benzoic acid, and Cytosine (viz. [Fe(Phen)(H2O)4]Cl3, [Fe(Phen)(BA)(H2O)3]Cl3 and [Fe(Phen)(BA)(Cyt)(H2O)2]Cl3, where synthesized and characterized. The complexes were synthesized in solution and characterized using physicochemical (conductivity measurement, Melting point, chloride test, solubility test, thermal analysis (TGA), and spectroscopic techniques (IR and UV-visible). Based on the analytical and spectroscopic data, octahedral geometries are assigned to the complexes. The in vitro antibacterial activity was tested on one Gram-positive Staphylococcus aureus (S. aureus) and one Gram-negative Escherichia coli (E. Coli) bacteria using the disc diffusion method. The complexes demonstrated appreciable activity against both pathogens. The antibacterial activity of the synthesized complexes showed less activity than the commercial antibiotic tetracycline against all the strains studied (S. aureus and E. coli). The percent activity of [Fe(Phen)(H2O)4]Cl3 complex showed better antibacterial activity than synthesized [Fe(Phen)(BA)(H2O)3]Cl3 and [Fe(Phen)(BA)(Cyt)(H2O)2]Cl3 complex in Gram negative (E. coli) bacteria. The results provide an insight to design and readily prepare task-specific metal-based drugs for interaction with particular bacterial strains.