In the realm of coordination chemistry, the manipulation of metal ion properties through diverse ligands is pivotal, encompassing the stabilization of various oxidation states and the adjustment of solvophilicity, electrophilic, and nucleophilic characteristics to tailor medicinal properties. This endeavor drives researchers to dedicate their time, energy, and resources towards the development of antibacterial agents with novel modes of action. In this study, two Ag+ complexes, [NO 3 Ag(2,2’-bpy)] and [NO 3 Ag(4,4’-bpy) 2 ], were synthesized employing 2,2’bipyridine (2,2’-bpy) and bis-4,4’-bipyridine(4,4’-bpy) 2 as ligands, respectively. The synthesis validity was confirmed through conductance measurements and spectroscopic analyses (FT-IR and UV/Vis), with the metal content determined via thermo gravimetric assessment. These analyses suggest a planar triangular geometry for the synthesized complexes. Subsequently, the in vitro antibacterial efficacy of these complexes was assessed against two Gram-positive bacteria (Staphylococcus aureus and Staphylococcu. epidermidis ) and two Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae), with their activities benchmarked against commercially available tetracycline. Notably, both complexes exhibited significant activity against all tested pathogenic strains, with [NO 3 Ag(2,2’-bpy)] demonstrating superior efficacy compared to the ligand, starting salt, and the second complex [NO 3 Ag(4,4’bpy) 2 ]. While the activities of these complexes were lower than that of the reference tetracycline, subsequent in vivo cytotoxicity assessments suggest their potential as antibiotic candidates, particularly against the tested bacterial strain.