Actually great distribution and drug resistance of microorganisms is one of our most concern and serious health problems. Infections from resistance bacteria are now common, and certain pathogens have developed resistant to several classes of antibiotics. Transition metal based drug represents a promising approach of antibacterial agent with potential application for inhibition of bacterial infectious diseases. Consequently, in this study relatively abundant and inexpensive element Iron (III) forms a new complex of the ligand 2,2’-bipyrdine and bitartrate [Fe(Bpy)(H 2 O) 2 (HTA)]Cl were synthesized. Varieties of analytical, thermal, and spectroscopic methods have been applied to characterize the structures of the complexes; in particular, FT-IR and UV-Vis performed. From IR and UV result both ligands, HTA 2 - and 2,2 bpy bonded to the metal center, so that octahedral complexes formed. The produced metal complex was further evaluated through the Disc Diffusion method for its in vitro antibacterial activity against Grampositive (Staph aurous, Staphylococcus Epidermdics) and Gram-negative (Escherichia coli, Klebsiella pneumonia) bacteria. The outcomes were compared with those of the frequently encountered commercial drug Tetracycline. It reveals small antimicrobial activity towards grampositive bacteria. The results are evident to antimicrobial activity of the complexes were predominantly effective towards strong Gram-positive bacteria (S aurous and S. Epidermdics). Prevailing, the complex can diffuse for those lacking strong cell wall. Generally, two octahedral complexes were synthesized; characterized and antibacterial activities were investigated