The present study intended to investigate the phytochemicals, antioxidant and antibacterial activities of petroleum ether, chloroform and methanol extracts of root of Rumex abyssinicus. Qualitative phytochemical screening of the crude extracts revealed the presence of phenolics, flavonoids, terpenoids, glycosides, Saponins and tannins. The antioxidant activity was evaluated using 2, 2-diphenyl-1picrylhydrazyl radical (DPPH). The total phenol content for all the crude extracts was determined using The Folin- Ciocalteu reagent (FCR) or Folin phenol reagent or folin-Denis reagent, also called the Gallic acid equivalent method. The highest total phenolic content was obtained in methanol extract (52.89% mg GAE/g), followed by chloroform extract (38.568 % mg GA E/g) and petroleum ether extract (37.486% mg GAE/g). All crude extracts of root Rumex abyssinicus exhibited antioxidant activities. The highest antioxidant activity was found in methanol extract (84.23 % in DPPH with IC50 value 30.44 ppm), followed by chloroform extract (79.46% in DPPH with IC50 value 55.6 ppm) and petroleum ether extract (61.41% in DPPH with IC50 value 78.43ppm). The antibacterial activities were examined using Agar disc diffusion method. All crude extracts showed antibacterial activities against gram negative bacteria (E. coli and K. pneumonia) and gram positive bacteria (S. aureus and S. pyogenes). The result showed that the crude extracts of root