This study was conducted in Mayteklet natural forest, Adiarkay district, North Gondar Zone, Amhara Regional State, to gain a comprehensive understanding of the species composition, structure, and regeneration status within this ecosystem. A five transect line sampling approach was employed, establishing 43 plots measuring 20 m x 20 m and 5 smaller subplots measuring 5 m x 5 m, thoroughly documenting the diversity of plant species. A total of 83 woody species belonging to 33 families and 58 genera were recorded, with Fabaceae and Moraceae identified as the most dominant families. Cluster analysis using R software revealed the presence of five community types in the Mayteklet natural forest. The density of mature woody plant individuals was found to be 2,460 stems per hectare, while sapling and seedling densities were 3,211 and 4,330 stems per hectare, respectively. The total basal area of the forest was calculated to be 41.5 m²/ha, providing insights into the overall biomass and productivity of the ecosystem. Regeneration data suggested that the Mayteklet natural forest is currently in good regeneration status overall; however, some species with low Importance Value Index (IVI) and limited or absent seedling presence may be at risk of local decline. Based on these findings, it is recommended that responsible authorities design and implement appropriate conservation strategies to address the needs of these vulnerable species, ensuring the long-term ecological integrity of the Mayteklet natural forest. Key Words/Phrases: - Community types, Species richness, Floristic composition, species diversity and Structure of Woody species