Antimicrobial resistance is one of our most series health problems. Infections from resistance bacteria are now common, and some pathogens have been become resistant to multiple classes of antibiotics. Transition metal based drug represents a promising approach of antibacterial agent with potential application for inhibition of bacterial infectious diseases. Consequently, in this study Iron (II) new complexes of the ligand bitartrate [Fe(HTA)(H2O)4]Cl and mixed ligand complex of 2,2’-Bipyridine [Fe(HTA)(Bpy)(H2O)2]Cl were synthesized. The structures of the complexes were characterized using various analytical, thermal and spectroscopic techniques, particularly using UV-Vis and FT-IR. Furthermore, the synthesized metal complexes were evaluated for their in vitro antibacterial activity against Gram-positive (staphylococcus aurous, Staphylococcus epidermidis) and Gram-negative (Escherichia coli, and Klebsiella Pneumonia) bacteria by Disc Diffusion method and their antimicrobial activity were compared with the standard commercial drug TTC. The final complexes showed good antimicrobial activity towards Staphylococcus empidermidis and Escherichia coli. Keywords: Bitartrate, 2, 2’-Bipyridine, mixed ligand complex, antibacterial activity, iron (II