Ethiopia is endowed with an enormous diversity of plants. However, the majority of these plants have not been scientifically investigated, particularly their medical value. Traditional knowledge on the use of plants as medicinal agents have been transferred from generation, as guarded secrets, through word of mouth, and scientific studies on these herbs have not been properly compiled. They are the richest bio-resource of drugs for traditional systems of medicine, modern medicines, food supplements, pharmaceutical intermediates, and chemical entities for synthetic drugs. Brucea antidysentrica is used traditionally to treat various diseases such as leprosy, wound, diarrhea, fever, eye disease, rabies, and tumor/cancer. The present study was intended to evaluate phytochemical screening, antibacterial as well as antioxidant activities of seed extracts of Brucea antidysenterica. The phytochemical screening was carried out by chemical test using standard procedure and their chemical composition was identified by GC-MS.. The highest total phenolic content, antioxidant and antibacterial activity was observed in ethanol crude extracts. The antioxidant activity of each crude and essential oil was determined using the DPPH assay , standard ascorbic acid shows the highest antioxidant activity, which scavenges 83.82353% of DPPH free radicals. The highest %inhibition was obtained from oil at 80 ppm concentration, which is 68.48% as compared to with petroleum ether and CuO/B.A and. In general, the result suggests that the seed of B.antidysentrica plant could be a potential source of natural antioxidants that could have great importance as therapeutic agents in preventing or slowing down the progress of aging associated oxidative stress related to degenerative diseases such as diabetes, cancer and cardiovascular diseases caused by lack of antioxidant capacity. Keywords: phytochemical screening, crude extract, essential oil, antioxidant, GC-MS analysis