The purpose of this study was to Examine Hydration status of among Gonji Kolela woreda U-17 male football project players. The study employed experimental research design. for this study 28 Gonji Kolela woreda U-17 year male football project players were included as the study sample, and purposive and comprehensive sampling grouped in to experimental group(EG ) N=14 and control group(CG) N=14. Both experimental and control group were engaged on 8-days proposed with fluid intake and without fluid intake two-three hour per session. However, the control group did not intake mixed water (lemon, salt and sugar) intake only 1-2 letters of water whereas experimental group intake mixed water the same letters. The selected hydration biomarker variables lemon, salt and sugar. Both groups had taken pre and post-testing and all the subjects participated in the morning the first urine execration and without any food and fluid intake but during sweat rate test during exercise both groups take fluids then measure their weight. The data collected from the study subject was analyzed using SPSS version 20 software by paired t-test with level of correlation -1.0 to-0.5 or1.0 to0.5 strong relationship,-0.5 to-0.3 or 0.5to 0.3 moderate,-0.3 to -0.1 or0.3 to 0.1 weak and -0.1 to0.1none or very weak relationship and significant 0.05. The results of this study showed that one or two lemon, two or four spoon sugar and half or one tea spoon salt mixed water fluid intake strong relationship both groups(EG and CG) than only water intake increases players hydration status, however weak relationship of CG. Based on this finding, it can be conclude that natural water with one lemon, four tea spoon sugar and one teaspoon salt mixes strong relationship than only water fluid intake on player’s hydration status through the required tests. Hence, break time fluid intake especially 1-lemon, 1-teaspoonsalt and 4- teaspoon sugar mixes water increases football players hydration status.