Access to safe water is one of the necessities for human beings for drinking and Hygiene Sanitation practices. Lack of safe and adequate water supply causes health risk and the situation is serious in developing countries like Ethiopia .Despite the drinking water supply in Addis Zemen town was from a borehole through pipes, a high incidence of waterborne diseases was frequently reported. The objective of this study was to assess the bacteriological and physicochemical quality of drinking water and hygiene-sanitation practices of the consumers in Addis Zemen Town. A total of 126 samples were collected randomly from boreholes, reservoir, taps, and household storage containers for bacteriological and physicochemical drinking water quality analysis from March to April 2022 for dry season and July to August 2022 for witer season, and the hygiene and sanitation practices of 30 customers were assessed using interviews. Total and fecal coliforms were anlysed using membrane filtration while selected physicochemical parameters were determined using standard methods. 80.9% (102/126) of drinking water samples were positive for total coliforms (TC), and 61.9% (87/126) of water samples were positive for faecal coliforms (FC) and did not satisfied WHO guidelines and Ethiopian standards for drinking water quality. There were statistically significant differences in both TC and FC coliforms among sampling sites and seasons. All physico-chemical parameters were satisfied values recommended by WHO except temperature and residual chlorine. The sanitary condition of water source and hygiene and sanitation practices of customers at household level were poor. Appropriate management of drinking water sources, proper disinfection of raw water with chlorine, and promoting good hygiene and sanitation practices are recommended to deliver safe drinking water to the consumers of the study area. Keywords: Bacteriological, hygiene- sanitation, physico-chemical, water quality,