Around 80% of the population uses plant based traditional medicine in Ethiopia. The primary goal of this study was to investigate and document medicinal plants used to treat human and livestock diseases in the Estie district. Ethnobotanical data were collected from January to March 2023 on trips made to six sites. In a total of 120 informants and 24(18 men and 6 women) key informants were selected through purposive sampling technique and other ordinary respondents were selected by lottery method. Primary data sources are the source for this study. The ethnobotanical data were collected through group discussion, semi-structured interview, field observation, and market survey. The data were analyzed by using descriptive statistics, preference ranking, direct matrix ranking, and fidelity level. A total of 120 MPs species in 97 genera and 52 families were collected and documented. From those, 105 MPs species were used for the treatment of human diseases, 35 MPs species were used for treatment of livestock diseases. Fabaceae, Solanaceae, and Poaceae were the most widely utilized families. MPs were collected from wild 77 (64.2%) species, home garden 23 (19.2%) species, crop field 9 (7.5%), and in both the wild and home gardens 11 (9.2%) species. Herbs constitute the highest category with 54 species (45%), followed by shrubs with 42 species (35%), trees with 16 species (13.3%), and climbers with 8 species (6.7%). The local communities mostly use leaves 89 (39.4%), followed by roots 57 (25.2%) and seeds 39 (17.3%). Fresh plant materials were used more frequently 139(61.5%) followed by dried 66(29.2%) and fresh or dried 21(9.3%). The most widely used method of preparation were pounding 60 preparations (26.5%), followed by squeezing with 29 preparations (12.8%), and crushing with 27 preparations (11.9%). Preparations were administered mostly by oral 119 (52.7%), followed by dermal 72 (31.9%), and fumigation 14 (6.2%). There was a high preference for Ficus palmata to treat wound infection. Eucalyptus globulus was the most multipurpose use plant. In general, the study area is abundant in MPs, which play an important role in the treatment of various human and livestock diseases. Encouraging traditional healers by providing training, professional supporting, and integrating their work with modern healthcare system were recommended. Keywords: Disease, Ethnobotany, Ethnoveterinary, Medicinal plant, Traditional healer