Despite the fact that household water storage containers are useful for making water reliable in locations with intermittent water supply, the deterioration of the water quality in the storage containers is a public burden that requires in-depth investigation. The aim of this study was to analyze the microbiological and physicochemical quality of drinking water from tap and household consumption level in Tana sub-city of Bahir Dar city. Microbiological and physicochemical analysis of the total of 208 water samples at 74 sampling points and sanitary surveying were conducted in randomly selected 74 tap water, service reservoir 5 and 129 household’s storage water container and analyzed. Water samples were examined for E. coli. Using membrane filtration method and the physico-chemical water quality parameters were measured on-site in the sampling locations. The data Analysis obtained from water quality analysis and survey results were imported MS excel, SPSS version 23 and one-way ANOVA, using the Tukey statistical analysis method, which is preferable when comparing three or more groups. The physicochemical water quality results revealed that the mean values of TDS and pH comply with the standard limits (500-100 mg/l) and (6.5-8.5) respectively for all sample groups in dry and wet seasons. Despite this, the mean temperature values of all water samples ranged from 22.70±0.74 0 C to 26.20±1.35 0 C, which was beyond the permissible limits (15-20 0 C). Furthermore, the mean values of turbidity measures were within the standards (<5 NTU) for dry season samples whereas all wet season samples did not comply with this except the mean values of household treatment unit samples (2.72±1.35 NTU). The microbial water quality analysis showed that the samples from home treatment units (64.8%) and customer taps (54.5%) had a lower risk (< 1CFU/100ml) microbial E. coli detection in the dry season while the free residual chlorine concentration was relatively higher in these sampling points. The Spearman’s correlation test indicated that there was a strong positive relationship between the E.coli presence and sanitation and hygiene conditions. Moreover, the sanitary risk scores ranged from low-risk to high-risk categories, with potential risk factors, such as improper storage container operation, and placement away from unsanitary conditions, poor hand washing practices during water collection, and infrequent at-home treatment methods. Thus, this study suggested interventions on awareness creation and promotions about safe water storage, handling, and treatment practices in households. Keywords: Household Storage Containers, Sanitary Inspection, Water Quality Parameters,