The cobalt doped bismuth ferrite nano particles are synthesized via sol-gel route using nitrate of iron and bismuth precursor in ethylene glycol as solvent and different amount cobalt ion as dopant. The prepared nanoparticles have been characterized by X-ray diffractometry, Fourier Transform Infrared spectrometry and BET. The X-ray diffractometry result demonstrates the synthesized powder having higher amount of cobalt ions has a rhombohedral crystalline structure with particle size of 31.2 nm. Cyclic voltammetry was used to evaluate the electrochemical behavior of the H2O2 at the modified nafion/BFO/GCE. Experimental parameters including, accumulation potential and accumulation time were investigated. The optimum cyclic voltammetric response was observed for 4 mg Co-doped BFO. Accumulation potential of -0.45 V and accumulation time of 300s were optimized. Besides, the novel non enzymatic sensor was also free from the interference of AA, Glu and Suc. And also the sensor for H2O2 determination showed good stability and sensitivity. Key words: Bismuth ferrite, Hydrogen peroxide and Sensors