There are over two hundred traits that are transmitted from generation to generation in humans, which is an interesting aspect of human genetics and known as hereditary traits. This genetic variation can be caused by migration, gene flow, and genetic drift. In this study, seven morphogenetic traits were selected for investigation, and the ABO blood group as well as the Rh (D) phenotype of study participants were investigated. The aim of this study was to find out the distribution of selected morphogenetic traits amongst the two ethnic groups of Amhara and Oromo to determine the association of each morphogenetic trait. Data was collected randomly by using lottery methods to select 384 participants. Selected morphogenetic traits were observed and recorded according to a common observable traits report sheet prepared by the researcher. Identification of blood groups was done by slide methods of agglutination by using anti-sera (anti A, B, and D) reagents. The collected data was analyzed with the aid of SPSS version-26 by descriptive stastics and regression analysis to find an association of morphogenetic traits with sex, ethnicity, ABO blood group and Rh factor. The investigated results showed that morphogenetic traits such as dangly or free earlobe, ability to tongue roll, straight little finger, straight shape of thumb, absence of dimple, straight hair line demarcation or non-widows peak, and right handedness had a high percentage prevalence in the study area of both ethnic groups. Morphogenetic traits such as free ear lobe or dangly, tongue rolling ability, straight shape of thumb, and facial dimple were most frequent in females than in males. Except for the type of handedness, chi-square analysis shows a significant association between morphogenetic traits and respondent sex (P < 0.05). In multivariate analysis of morphogenetic traits with blood group distribution, the subject with blood group O had significance association with earlobe attachment while individual with blood group B and AB had significance association with tongue rolling and shape of thumb at (P<0.05). In crude odd ratio, earlobe attachment, show a significant association with ethnic group at (P < 0.05). In blood group distribution, blood group O is the most dominant, followed by blood groups A and B, and the least distributed blood group AB. The subject with the blood group “O” has a high prevalence of free earlobes and the ability to tongue roll. There was a high percentage of Rh+ positives and a low percentage of Rh negatives in the study area. In terms of morphogenetic traits and blood group distribution, the high school and health office, in collaboration with higher education, were better to study with a larger sample size for advancements in morphogenetic traits and blood group distribution to understand association and variation between population.