Access to safe drinking water and sanitation is a global concern. Water quality deterioration is a big issue in many countries water supply system, which may be a result of many interconnected physical, chemical, and biological factors. The problem is more severe in developing countries, like Ethiopia Therefore, the purpose of this study is to assess the spatial and seasonal drinking water quality variation in Woldia town, Amhara Regional State, Ethiopia. The study was conducted from December 2020 to July 2021. Two rounds of water samples were taken in dry and wet seasons from each sample site with a total of 220 water samples. As per the principle of WHO sampling points selection, 28 sampling points ;that is, water sources (n=7), reservoirs (n=3) and distribution points (n=18) were taken to analyze physicochemical and bacteriological parameters. Data was analyzed using SPSS version 23. The study used descriptive statistics such as mean and standard deviation while inferential statistics such as Pearson correlation and analysis of variance (ANOVA) were used to see the significant association between parameters and significant difference among sampling points at p<0.05 respectively. The result showed that except total hardness, free residual chlorine and water temperature all selected physicochemical parameters were found within the acceptable limit of WHO and Ethiopian standards in both seasons. On the other hand, the bacteriological test results revealed that majority of sampling points were above the standards set by WHO and Ethiopian guidelines. In the distribution reservoir, except temperature and total hardness all physicochemical and bacteriological water quality parameters recorded in distribution reservoirs met the World Health Organization (WHO) and the Ethiopian Water Quality Guidelines. The variability analysis (ANOVA) test revealed that there were no significant differences for me an values of majority of physicochemical and bacteriological parameters at sources both spatially and temporally; nevertheless, at household taps there was significant differences among majority of sampled sites spatially at p<0.05 significant levels. Finally, the overall water quality status of the study area by using WQI indicated that the individual water samples varied from 5 to 232; however, significant numbers of sampling sites were ranged from slightly polluted to heavily polluted status. Therefore, subsequent monitoring of drinking water quality is needed to ensure community health and sustainable water quality. Keywords: Water Quality, spatio-temporal variation, physicochemical and bacteriological parameter, water quality Index, Woldia town.