The objective of this study was to find out the effect of calisthenics exercise on some selected physical fitness qualities and skill performance at Bahir Dar University u-17 female Handball project players. The study applied a quantitative research approach and employs an experimental design to attain the intended objectives. For this study, the researcher has selected all 20 female Handball players who participate in the Bahir Dare University u-17 female Handball project. Were participated by using a comprehensive sampling technique and were randomly assigned into two equal groups, the Experimental group (N=10) and the Control group (N=10). During 8 week intervention, the experimental group formed calisthenics exercises for three days and each session lasted 60 minutes including warm-up and cool-down exercises. The control group participated in only a regular handball training program. The data were measured on passing accuracy, speed dribble, agility, and muscular strength on two occasions first before administration of calisthenics exercise as a pre-test and second after 8 weeks of the calisthenics exercise as a post-test. The data collected from the subject of the study were analyzed by using SPSS version 23 software by paired sample t-test and independent-sample t-test with a significance level of 0.05. The analyzed data showed that calisthenics exercise significantly improved passing accuracy, speed dribble, agility, and muscular strength in the pre-post test of EG at (p<0.05) But no significant differences were found in all of the variables in CG at (p>0.05). In addition, as it was assessed by the independent t-test the post-test passing accuracy, speed dribble, agility, and muscular strength in EG were significantly improved than the post-test result of CG (p<0.05). Based on this finding, it can be concluded that the eight-week calisthenics exercise helps to improve agility, muscular strength, passing accuracy, and speed dribble of u-17 female handball project players.