Background-: Episiotomy is surgical obstetric procedure that performed in the second stage of labor, which divides the tissues of the perineum in order to enlarge the vaginal outlet and facilitate delivery of the baby. World Health Organization recommends the practice of episiotomy to be below 10% but in Ethiopia the practice of episiotomy specifically Public Health Institution AkakiKality in Addis Ababa was 35.2%. Despite actual practice of episiotomy with its high magnitude, there is no similar research done at Debre Tabor Comprehensive Specialized Hospital. Objectives- The objective of this study is to assess the proportion of episiotomy practice and its associated factors in Debre Tabor Comprehensive specialized hospital, South Gondar zone, Amhara region, Northwest Ethiopia in 2021 G.C. Method- Institutional based cross-sectional study was conducted among 590 study participants; from April 15 to July 2, 2021 G.C .The Study participants were selected by using systematic random sampling technique. As the health management information system, registration book shows in the last six months averagely there were 450 vaginal deliveries per month in Debre Tabor Hospital. A systematic sample was used to select samples from 900 mothers who will give birth vaginally in the study period. As the sample size is 590, the interval to select mothers is two. Therefore, every other mother who gave birth vaginally was interviewed in this study. Data was collected using structured interview based questionnaires. Simple binary logistic regression analysis to identify factors associated to identify candidate variables for multiple binary logistic regressions. Variables in simple binary logistic regression with P-value≤0.25 is entered in multiple binary logistic regressions to identify factors associated with episiotomy practice with P-value less than 0.05 at 95% confidence interval. Result: In this study the proportion of episiotomy is high which is found 18.3%. Primiparus, second stage of labor 1-2 hour duration and mothers were 2-4 pregnant are significantly associated factors with episiotomy practice. Conclusion: proportion of episiotomy practice is high based on WHO recommendation. Factors which associated with episiotomy practice were Primiparas, second stage of labor 1-2 hour duration and mothers are 2-4 pregnant.