Background: Pain is a global challenge that has complexity and a multidimensional phenomenon. According to International Association for the Study of Pain (IASP), the newest definition of pain is a distressing experience associated with actual or potential tissue damage with sensory, emotional, cognitive and social components. Pain has detrimental effect on pediatrics but most HCPs in both developing and developed countries have limited level of practice in pain management. Objective: To assess practice of health care professionals in pediatrics pain management and associated factors in governmental referral hospitals at Bahirdar, Ethiopia 2020. Method: A cross-sectional institution-based study was conducted on HCPS working in TGSH and FHSHs in Bahirdar. The data collection was undertaken from August 1 to 30, 2020 by prepared questionnaire. It was cleaned, entered and analyzed by SPSS version 23. The study population were HCPs in pediatrics department and pharmacy units in governmental referral hospitals at Bahirdar with a sample size of 155. After analysis frequency and percentages were used to summarize the finding and significance of determinant factors were tested using binary Logistic regression and Odds Ratio & P <= 0.05 was considered significant. Result: The results of the study showed that 60.6% of HCPs had good practice and 39.4% had poor practice in pediatrics pain management. Regarding their knowledge the result showed majority of HCPs had good knowledge towards pediatrics pain management. It was found that, knowledge was significantly associated to pediatrics pain management practice with 0.025 P- value. Conclusion and Recommendation: The finding emphasized the need to improve practices of HCPs on management of pain in children by improving the knowledge level of HCPs. Majority of HCPs had no in-service training, pain management protocol, previous course on pain management and pain assessment tools. The findings also showed use of WHO pain management ladder was limited. This study recommends development of mechanism to provide HCPs in-service training on pain management of children, incorporate content into the undergraduate courses and avail pain management protocol in the hospitals to improve pain management practices in pediatrics. Key words: Pain, pain management, HCPs, pediatrics, practice, knowledge, attitude