Background: Health care associated infections also known as hospital acquired infections (HAIs), are infections that are not present (may be incubating) at the time of admission but acquired during the delivery of health care. Though, HAIs are major public health problem in hospitals worldwide, the prevalence is two to three folds higher in developing countries compared to developed countries. Health care worker’s fomites are highly prone to bacterial contamination from the health care setting and are considered as potential sources for HAIs. However, there is scarcity of data that shows the magnitude of bacterial contamination and antimicrobial susceptibility pattern of isolates from health care workers (HCWs) fomites in Ethiopia.