The electrooxidation behavior of amoxicillin was studied at Nobel poly(diaquabis(1,10-phen anthroline)copper(II)chloride) (poly(A2P2CuC) polymer film synthesized potentiodynamically from a complex monomer modified glassy carbon electrode is reported for detection of amoxicillin. Deposited poly(A2P2CuC polymer film on the surface of glassy carbon electrode was characterized using cyclic voltammetry and electrochemical impedance spectroscopy using [Fe(CN)6] 3-/4- as a probe. In contrast to the unmodified glassy carbon electrode, appearance of an oxidative peak with over twofold current at a low potential at the poly(A2P2CuC/GCE) showed catalytic property towards oxidation of amoxicillin. This might be attributed to the twofold increased effective electrode surface area, and improved conductivity of the electrode surface. Better correlation of the oxidative peak current with the square root of scan rate (R2 = 0.99779) than with the scan rate (R2= 0.96953) indicated that the oxidation reaction of amoxicillin at the polymer modified electrode is predominantly diffusion controlled electrode process. The slop, 0.58, in the plot log Ip vs log v further confirms as the electrode process is diffusion controlled. At phosphate buffer solution pH of 5.5 and optimized square wave voltammetric parameters, current response of poly(A2P2CuC)/GCE showed linear dependence on concentration of amoxicillin in the range 2.0 × 10-6 – 1.0 × 10-4 M with detection limits, and limit of quantification of 1.15 × 10-8 and 3.85×10– 8M, respectively. Spike recovery in tablets in the range 98.90-101.95%, and serum sample in the range 101.37-102.20%; interference recovery in tablets with an (%RSD) in the range 0.00-4.51%, and in serum in the range 0.00-2.10; and a value with an error under 1.14% for quartet measurements recorded every two hrs interval in a day added with the wide dynamic range and low LoD validated the method for determination of amoxicillin in real samples. Keywords: amoxicillin; antibiotics; modified electrode; potentiodynamic electropoly merization