Genetically modified organisms have been accessible for commercial purchase since 1990s; allowing producers to increase yield through genetic engineering that produces herbicide, insect and disease-resistant varieties of organisms. However, Consumers all across the world have a limited knowledge and even unfamiliar with it. Controversy on GMO use is still there. This study aimed to investigate the knowledge, attitude and acceptability of GMOs among teachers of Bahir Dar University. Institution based cross sectional study was conducted from September 2020 to June 2021. Data were collected through distribution of questionnaire for a total of 292 teachers and 223 were completed and returned. Descriptive statistics, bivariate and multivariate logistic regressions were used to analyze the collected data with SPSS V.23. Thirty five (15.7%) of the participants were science, 13.0% were food and chemical engineering, 19.3% were agriculture and environmental science, 13.5% were humanities and 38.6% were medicine and health sciences teachers. Most of the participants 153 (68.6%) had masters degree. More than 40.4% of them had 6-10 years of experience in their profession. The majority 215 (96.4%) were Orthodox religion followers. Two hundred two were heard about GMOs. It was found the dominant 143 (70.79%) of the participants had good level of knowledge. Logistic regression showed that Agriculture and environmental Sciences teachers knowledge about GMOs was 6.238 time more likely than medical and health science teachers [AOR= 6.238, CI (1.367-28.462), P =0.018]. Of the respondents, 55 (27.23%) had positive attitude towards GMOs. Eighty seven (43.07%) of them accepted GMOs use whereas 115 (56.93%) did not. Science teachers accepted GMOs 0.204 times less likely than medical and health science teachers [AOR=0.204, CI (0.072-0.576), P =0.003]. Agriculture and environmental science teachers also accepted GMOs 0.210 times less likely than medical and health science teachers [AOR= 0.210, CI (0.082-0.538), P=0.001]. Teachers having negative attitude for GMOs accepted 0.109 times much less than teachers who had positive attitude towards GMOs [AOR= 0.109, CI (0.048-0.248), P=0.000]. Environmental and biodiversity damage, cancer and religion were their main concerns about GMOs. It could be conclude that participants of this study were well aware on GMOs with less positive attitude and acceptance. Further studies would be conducted with larger sample size and would have to discuss on the use and disuse of GMOs in Ethiopia with the Health care professionals, geneticists, biotechnologists, ecologists, religious leaders, economists and other stakeholders. Key Words: Genetically modified organisms, Teachers, Knowledge, Attitude, Acceptance