Maize is staple food and the most cultivated crop in Ethiopia. However, its yield is very low partly due to use of improper spacing’s Thus, an experiment was conducted at Bahir Dar, Amhara Regional State of western Ethiopia during the 2019/2020 cropping season. The objective of this study was to investigate the effects of spacing between rows on growth, yield and yield component of maize, (Zea mays L.) Variety BH-540.The experiment was arranged in a factorial combination of the four inter-row spacing (40, 70, 80, and 90 cm) and three intra-rows (35, 40 and 45 cm) spacing. Also the experiments were laid out in complete randomized design (CRD) with three replications by using Analysis of variances (ANOVA) for the data recorded were conducted using the SPSS (Version 20). The results showed that there was significant (P< 0.01) main effect of interrow spacing on the growth, yield and yield components of maize. Leaf area, number of ears per plant, 100 kernels weight per ear, above ground dry biomass yield and number of kernels per ear significantly increased with increased the interaction of inter and intra-row spacing. The maximum leaf area (1002.55cm2 ), number of ear per plant (1.60), 100 kernels weight per ear (21.11g), above ground dry biomass yield (620.60g), and number of kernels row per ear (14.20), were obtained from 90 cm inter-row with 45 cm intra-row spacing combinations. In addition to there were significant difference (P < 0.01), due to the main effects of intra-row spacing was observed on stand count and ear weight. Maximum stand count (100.00 %), and ear weight (276.60g), were formed from 90 cm inter-row with 45 cm intra-row spacing interaction. There was significant effect (P < 0.05), of intra-row on above ground dry biomass yield per hectare. The maximum above ground dry biomass yield per hectare (27.54 t ha-1 ), was obtained from the interaction of 70 cm inter-row with 35 intra-row spacing. There was highly significant interaction effect (P < 0.01), of inter-row by intra-row spacing on grain yield per plant, and grain yield per hectare. The maximum grain yield per plant (274.00g), was obtained from the interaction of 90 cm inter-row by 45 cm intra-row spacing. While grain yield per hectare (19.94 t ha-1 ), was obtained from 70 cm inter-row by 35 cm intra-row spacing. In general, significantly, higher grain yield per hectare and above ground dry biomass yield per hectare with highest economic benefit (356570 birr ha-1 ), were obtained from 70 cm inter- row x 35 cm intra-row. It could therefore be concluded that farmers in the study area can use spacing combinations of 70 cm inter- row x 35 cm intra-row spacing for enhancing maize grain yield and above ground dry biomass yield. Key phrases: Crop, Leaf area, Maize ecology, Phenology, Plant density