Congenital anomalies and genetic conditions affect 7.9 million children worldwide and 94% of all births affected with congenital anomalies occur in law-and middle-income countries. Genetic counselling, the process of helping people understand and adapt to the medical, psychological, and familial implications of genetic contributions to the disease, is an effective method of reducing a country's incidence of congenital anomalies and genetic disease. The physicians’ unfamiliarity with specific requirements of genetic counseling may impair the quality of care for clients. The purpose of this study was to assess the level of knowledge, attitude and practice among healthcare professionals towards genetic counselling in North West Ethiopia from January to august, 2020. An institution based cross-sectional design was conducted using structured and pretested questionnaire. Samples of 403 professionals selected from seven healthcare settings by stratified random sampling technique were included. Data was cleared, coded and entered into Microsoft Excel and then transported to SPSS version 21 software for data analysis. Descriptive statistics were used to report frequencies while bivariate and multivariate logistic regression analyses were employed to describe the association between dependent and independent variables. The study revealed that 46.2%, 58.8% and 25.8% of health care professionals were found to have good knowledge, attitude and practice, respectively. This implies that out of the total scores 11,9 and 13 (knowledge, attitude and practice respectively), health care professionals are found to have mean knowledge score of 4.6 ± 2.29, mean attitude score of 5.13 ± 2.53 and mean practice score of 4.01± 2.09 .Educational level with BSC (AOR=2.2; 95% Cl= (1-4.4);p=0.03) and post-graduate (AOR=7.8; 95% Cl= (3-20);p<0.001) , type of profession i.e. GPs (AOR=5.2; 95% Cl= (2.3-11.8); p<0.001) and Specialists (AOR=7.2; 95% Cl=(2.5-20.8);p<0.001), work experience with 16 years and above (AOR=1.2; 95% Cl=(0.5-2.3); p=0.046) and age group that have 51 years old and above (AOR=2.6; 95% Cl= (0.9-7.5); p=0.01) were factors significantly affecting healthcare professionals’ level of knowledge on genetic counselling. It was also indicated that educational level ( postgraduate (AOR=6.6; 95%CI =(2.6-14.1); p<0.001) , BSC (AOR=2.7; 95%CI=(1.51-5); p=0.01) and the type of profession in which specialists (AOR=2.4;95%CI=(0.9-6.4); p=0.02) and GPs (AOR=2; 95%CI=(0.9-4.6); p=0.04) significantly affected the level of attitude within the group whereas only the type of profession had significant association with their previous practice of genetic counselling