Dietary protein supplements offer a convenient way to ensure that athletes consume quality protein in their diet and meet their protein needs. The research focus over recent years has been to determine whether different types of protein have varying effects on the physiological, hormonal and immunological responses to different type of training. Isolate of soy protein and whey protein are commonly used protein sources supplements that certainly provide adequate amounts of protein in the diet for better exercise performance. Therefore, in this study, the researcher want to investigate the effect of Whey and Soy protein supplements on men athlete’s physical fitness qualities in reference to Debre Markos University Handball team. Comprehensive sampling was used to to select samples. Twenty six male subjects were randomly assigned to two experimental and one control group. Experimental groups were taken 25g isolate whey protein for EWG and 25g isolate soy protein to ESG for eight weeks after each training program. However, the control group did not taken any protein types. All data were analyzed using t-test and one-way measures analysis of variance. Twenty-six participants were completed the eight week study. Subjects in the EWG and ESG were shown significant difference between means in the strength and power than the CT group. Except EWG no significant difference between means were observed in the endurance performance in the ESG and placebo group after the experimental period. Key words: Handball team, Whey protein supplement, Soy protein Supplement,