The high cost of chemical coagulants and their accessibility for water treatment makes most people in rural community to resort to readily available surface water which are usually of low quality exposing them to different water-borne diseases. It is in this light, this research was conducted to assess the effectiveness of Papaya seed extract for the removal of turbidity and total coliform in raw water sampled from Tulte River which serves as potable water for the society. Jar test experiment was carried out through three type’s coagulant extraction methods for treatment of high (392.7NTU) & medium (94.2NTU) turbidity level to obtained optimum operation condition of dose, pH and mixing time plus suitable extraction method. Those optimized parameters of dose and agitation time were then applied in household level of water treatment through manual coagulation (coagulation by hand) in order to qualify its effectiveness. Coagulation test revealed that, superior total coliform removal of 96.32% through defatted salt extract and gave residual turbidity of 4.25NTU which is acceptable based on WHO drinking water standard. Overall, finding of this research suggest the potential of Papaya seed extract as bio-coagulant for turbid surface water treatment.