JavaScript is disabled for your browser. Some features of this site may not work without it.
| dc.contributor.author | GUALE, MEKONNEN | |
| dc.date.accessioned | 2020-10-08T07:18:26Z | |
| dc.date.available | 2020-10-08T07:18:26Z | |
| dc.date.issued | 2020 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/11321 | |
| dc.description.abstract | Environmental pollution is growing rapidly due to frequent release of hazardous substances. Researchers are always investigating new, low-cost technology and eco-friendly tools and / or methods capable of reducing or eliminating hydrocarbon pollutants from soil environments. For such applications, biological agents, most likely bacteria, have great potential to effectively transform and detoxify hydrocarbon pollutants. Therefore, this research was designed to isolate and characterize potential bacteria for the biodegradation of hydrocarbon-derived pollutants. For those research activities, the soil samples were collected from three aromatic hydrocarbon (benzene, kerosene, naphthalene, diesel )contaminated sites: Woira Sefer, Amanuel Sefer, and Akaki Mender old garages. Then, the samples were serially diluted and spread on selective media (Bushnell Hass Mineral Salts Agar Medium) designed for the cultivation of hydrocarbon degrading bacteria. Different methods such as physiological, chemical, biochemical, biolog, quantitative analysis were employed to isolate and characterize the bacteria. The result showed that bacteria were capable to grow on various hydrocarbons like Kerosene, Naphthalene and Diesel (KND)and nine different bacterial genera provisionally identified as Pseudomonas aeruginosa, Providencia rettgeri, Stenotrophomonasmaltophila, Escherichia coli, Staphylococus sciuri, Bacillus thuringiensis/cerus, Exiguabacterium aurantiacum, Acinetobacter lwoffii, and Acinetobacter beijerinckii were isolated from the above listed sites. In this study, the efficiency of hydrocarbon (KND) degradation of the individual bacterial cultures was determined quantitatively upon gravimetric analysis and the isolated bacterial culture of pseudomonas aeuroginosa achieved maximum diesel degradation while Bacillus spp., Pseudomonas aeruginosa and Acinetobacter beijerinckii were responsible for maximum kerosene degradation. Maximum degradation of naphthalene was attained by Providencia rettgeri and Pseudomonas aeruginosa. It is evident from this study that hydrocarbon degrading organisms are ubiquitous in the environment and they can be isolated from hydrocarbon polluted sites (like garages and fuel station areas).The study also suggests that the use of indigenous isolated bacteria could be considered as an alternative technology for effective and ecofriendly technology for the degradation of hydrocarbon pollutants. | en_US |
| dc.language.iso | en | en_US |
| dc.subject | Chemical Engineering | en_US |
| dc.title | ISOLATION AND CHARACTERIZATION OF HYDROCARBON DEGRADING BACTERIA FROM HYDROCARBON CONTAMINATED SITES: MICROBIAL BIOREMEDIATION PERSPECTIVE | en_US |
| dc.type | Thesis | en_US |
