This study examines the effect of working capital management on firm profitability in large manufacturing companies in Amhara region. In light of this objective, the study adopted explanatory research design and quantitative research approach. Additionally, the study also used purposive sampling technique to select sample. The regression results using random effect model showed that average collection period, inventory conversion period and current asset to total asset ratio had statistically significant negative effect on profitability. The study result also, average payment period had statistically significant postive effect on profitability of the sampled firms. Contrary to the hypothesis cash conversion cycle has statistical postive significant effect on profitability. However, current liabilities to total asset ratio had statistically postive insignificant effect on profitability. In general, the study concludes that both firm specific and macroeconomic facto that affects working capital management this effect also affected the profitability in Amhara region large manufacturing share companies.