Dandruff is a common disorder affecting the scalp condition caused by yeast Pityrosporum. Dandruff can‟t be completely eliminated but can only be managed and effectively controlled. A shampoo is detergent containing surfactant (i.e. surface active material) in a suitable form which when used under the specified conditions will remove surface grease, dirt, and skin debris from the hair shaft and scalp without adversely affecting the user. Various anti-fungal agents are employed in hair care preparations for the treatment dandruff. These products show many side effects like loss of hair, increased scaling, itching and irritation. Hence the project concerned to formulate natural anti-dandruff shampoo which is effective in terms of safety and treating the dandruff condition better than the chemical based anti-dandruff shampoo. Natural anti-dandruff shampoos were formulated using natural plant based ingredients like Lemon juice and Aloe Vera gel and other ingredients for preparing base shampoo. The formulated shampoos were subjected to evaluation parameters like pH, viscosity, Percentage of solids contents, conditioning action, cleansing action, Foaming ability and foam stability, anti-fungal activity test, eye and skin irritancy and physiochemical evaluation. The production of anti-dandruff shampoo was performed in three different mixer‟s speed at revolution per minute (rpm) of 100, 200 and 300 with different amount of sodium chloride (10g, 15g and 20g). It was found that 300rpm and 15g of NaCl are the desired speed and amount of salt respectively to formulate the anti-dandruff shampoo. Based on the chemicals involved, Different formulations of anti-dandruff shampoo (F1, F2 and F3) were made. Out of the three formulations, formulation one enhanced desired pH value = 5.87 and viscosity = 900cp. These were subjected to safety studies on animals, such as eye irritation test and skin sensitivity test. From these formulations, F1 exhibited good safety without any irritation and sensitivity. Besides, formation one was found good conditioner for hair. Stability studies for a period of one month were conducted for F1 formulation showed negligible changes in its physicochemical properties. All formations were good for anti-fungus activities.