The demand for renewable energy is increasing globally. Microbial fuel cell (MFC) systems represent a new promising technology that produce electricity while simultaneously treating wastewater. Brewery wastewater is one of the major pollutants in Ethiopia. The main purpose of this research was the utilization of brewery wastewater for production of electricity and simultaneous treatment using microbial fuel cell. A double chamber MFC was assembled with graphite electrode as anode and cathode, Nafion membrane for proton exchange, copper wire and different resistors were used. The MFC with 4000, 3000, 2000, 1000 and 500 mg/L chemical oxygen demand (COD) was inoculated with 150 mL mixed-culture of well-mixed sludge sample used anaerobically in the anode chamber (1.15 L). Potassium permanganate (KMnO4) at 0.3 M, 0.5 M and 0.8 M concentration was used aerobically as a final electron acceptor in the cathode chamber (1.15 L). In the alternative, instead of potassium permanganate, air was used in the cathode chamber as the final electron acceptor. The MFC bioelectrochemical batch process was operated for 20 days. Voltage and current generation were measured by Voltmeter and Ammeter. A maximum voltage of 1060 mV was obtained at 0.5M KMnO4 and 1000 mg/L COD on the 15th day. Based on the polarization curve, the maximum power density and current density were 682.83mW/m2 and 1.54mA/cm2 respectively with 200Ω external resistor for 0.5M KMnO4 and 1000 mg/L COD concentration. The COD removal efficiency of MFC was 45-67% at 0.5M of KMnO4 and 21-34 % when air was used as the electron acceptor. The maximum removal efficiency was obtained when potassium permanganate was used as the final electron acceptor in the cathode chamber. The results of this thesis demonstrated the potential of producing electricity from Ethiopian brewery wastewater and also treating the waste at the same time.