Forests play an enormous role in combating climate change, the most pressing global issue currently, by sequestering and storing carbon from the atmosphere through photosynthesis. This study was conducted to estimate total carbon stock of the dry Afromontane forest type of Zafnigus Forest and its implication for climate change mitigation. A grid method was used to identify each sampling point through Global Positioning System (GPS) and nested circular plots having 11.3m radius (401m2) and 8m radius (201m2) were employed for this study. All trees within a plot which have diameter at breast height (DBH) ≥5cm were recorded and its biomass was calculated by allometric equation. Walkley Black method was used to estimate soil carbon stock and data analyzed by SPSS version 20. Results revealed that the total carbon stock of the forest was 325.48 tha-1 whereas trees store 194.85 t/ha, litter, herbs and grasses (LHGs) 2.01 tha-1 and soil 128.62 t/ha (up to 30cm depth). Soil organic carbon was different in land use type of which 128.62, 94.72 and 38.42 tha-1 forest, cultivated land and grazing land respectively. Altitude was affect the carbon stock distribution in all carbon pool which was determined in this study. The lower parts of altitude had high carbon stocks in all carbon pool while the upper and middle parts of altitude due to the absence of tallest trees with maximum DBH, density, age, size, branches, management activities at the edge of the forest and decomposition rate. The result of study showed a significant variation in carbon stock in all carbon pool except litter carbon pool along altitudinal range at 95% confidence interval for the case of tree carbon stock (P=0.045); SOC (P=0.021, P=0.038 and P=0.016) comprised at 0_10cm, 10_20cm and 20_30 cm depth respectively and LHGs (P=0.194). Based on the result of this study the distribution of carbon was highly affected by forest density, height and DBH of tree in all carbon pools and the variation of altitude have an impact. The study revealed that tree biomass was the major reservoir of carbon followed by soil organic carbon and litter biomass. However, further research on the rate of carbon storage estimation and develop species specific allometric equation model should be done. Keywords: Zafnigus forest, Carbon stock, land use comparison, altitude and Climate change mitigation.