Khatasa protected forest is one of the protected forests found in Awi Zone and protected since 1994. Study on large mammal diversity, abundance and distribution in Khatasa protected forest was conducted from August 2018 to February 2019. The study area was divided into three habitats such as: Riverine forest, Dry Afromontane forest and Plantation forest habitats based on vegetation type during the preliminary survey. Representative sample sites were taken from each habitat type and surveyed randomly using line transect method. Diurnal large mammal species were identification through direct observations but, indirect methods were used for the survey of nocturnal and elusive species. Data collected from the study area were analyzed by using Shannon diversity index, Chi square test and Pair-wise ranking. In this study 13 large mammal species were identified and recorded from Khatasa Protected Forest (KPF). From this recorded large mammals one albino individual of Colobus guereza (fully White in color) was identified in the study area. Riverine forest had the highest diversity index (1.979) and evenness (0.75), whereas Plantation forest had the least diversity index (1.502) and evenness (0.722). Simpson similarity index (SI) of large mammal species among three habitats in the study area was 0.73. The abundance of large mammal species recorded in the different habitat types were significantly different χ2=1244.642, df = 12 p = 0.001 The abundance of large mammals were significantly different (χ2=1253.598, df = 12, p= 0.001) between wet and dry seasons. The relative abundance of large mammal species in habitat Riverine were (49.0%), Dry Afromantane (37.4%) and Plantation (13.6%). Colobus guereza was the most distributed and relatively abundant (34.69%) compared to the total recorded species in each habitats. Overgrazing, deforestation, agriculture and conflict were the major identified threats of large mammals in the study area. Khatasa protected forest is potentially rich in wildlife maybe the suitability of habitat. Currently, the area is suffering from different human activities. Administrative units and all othe