Abstract The present study reported the green synthesis of silver nanoparticles using aqueous khat (Catha edulis) leaf extract as a reducing agent. Plant extract is easily available, generally safe and nontoxic. They consist of phytochemicals such as Alkaloids, flavonoids, protein, tannins, Glycoside, and phenols. These constituents are responsible in the reduction of silver ions into silver nanoparticles. The synthesized nanoparticles were characterized by UV-vis spectroscopy, Fourier transform infrared spectroscopy, x-ray diffraction, and scanning electron microscopy. The formation of AgNPs were indicated by the color change from colorless to reddish brown. UV-Visible spectroscopy showed surface plasmon resonance (SPR) of the AgNPs at 428 nm. FTIR spectra analysis indicated the presence of biomolecules as reducing and stabilizing agents in the synthesized of silver nanoparticles. XRD analysis reveals that the synthesized AgNPs crystalline and exhibit a face-centered cubic structure. Additionally, antimicrobial activity of the AgNPs against Escherichia coli, Kleibsiella pneumonia, Staphylococcus aureus, and Streptococci pyrogens was evaluated based on the inhibition zone using the disc-diffusion assay. In conclusion, the synthesis of AgNPs with khat leaf extract is simple, rapid, environmentally benign and inexpensive. Moreover, these synthesized nanoparticles exhibit significant antibacterial activity. Keywords: AgNPs, Antimicrobial activity, Catha edulis, Green synthesis