ABSTRACT Background: Health care associated infections also known as hospital acquired infections (HAIs), are infections that are not present (may be incubating) at the time of admission but acquired during the delivery of health care. Though, HAIs are major public health problem in hospitals worldwide, the prevalence is two to three folds higher in developing countries compared to developed countries. Health care worker’s fomites are highly prone to bacterial contamination from the health care setting and are considered as potential sources for HAIs. However, there is scarcity of data that shows the magnitude of bacterial contamination and antimicrobial susceptibility pattern of isolates from health care workers (HCWs) fomites in Ethiopia. Objective: To determine the bacterial contamination and antimicrobial susceptibility pattern of isolates from HCWs fomites at Felege Hiwot Referral Hospital (FHRH), Ethiopia. Methods: A cross-sectional study was conducted from February 2017 to April 2017 from 422 HCWs fomites surface samples using convenient sampling techniques by simple-rinse method. Nutrient broth moisten sterile cotton swab used to rub the fomite. The swab was aseptically placed in 1 ml tryptic soy broth and diluted in 9ml of normal saline, then inoculated to plate count agar and incubated. Colonies were counted and calculated in terms of CFU/ml. Bacterial isolation and antimicrobial susceptibility testing were done following standard bacteriological techniques. Demographic and other explanatory variables were collected by face-to-face interview using structured questionnaire. The data from the finding was coded, entered and analyzed using Statistical Package for Social Science (SPSS) version 23. Descriptive statistics were used to get summary values. Binary logistic regression analysis was computed to see association between variables. A P-value < 0.05 was considered as statistical significant. Result: Overall, 243 (57.6%) of fomites were contaminated with aerobic bacteria colony count >5 CFU/ml. The highest contamination was found in mobile phones 165 (59.2%) with the isolation rate of 103 (62.4%) (P = 0.006). Differences, in field of specialization (P=0.05), working wards (P<0.001) and laundry washing of white coats (P=0.015) of HCWs were significantly associated with bacterial contamination. Coagulase negative Staphylococcus spp. (44%) was the leading isolates followed by S.aureus (32%) and IX K.pneumoniae (10.2%). Moreover, K.pneumoniae and E.coli showed 100% and 87.5% resistance to ampicillin and cotrimoxazole, respectively. Multidrug resistant was seen in 88.9, 92.6 and 100% of S.aureurs, K.pneumoniae and E.coli isolates, respectively. Conclusions: Bacterial contamination of HCWs fomites is a major problem in the study area. Multiple drug resistance of isolates is alarmingly high in both Gram positive and Gram negative bacteria. Therefore, health care workers of the hospital need to implement proper handling of fomites to reduce contamination. Keywords: Health care worker fomites, Bacterial contamination, Antimicrobial susceptibility, Felege Hiwot Referral Hospital, Bahir Dar, Ethiopia.