Performance evaluation of laboratory diagnostic methods for intestinal parasites among school children at rural Bahir Dar, Northwest Ethiopia

Birtukan, Bayayibign

dc.contributor.author	Birtukan, Bayayibign
dc.date.accessioned	2019-06-04T04:16:29Z
dc.date.available	2019-06-04T04:16:29Z
dc.date.issued	2018-09
dc.identifier.uri	http://hdl.handle.net/123456789/9514
dc.description.abstract	Abstract Background: -The prevalence of intestinal parasites in developing countries is high due to poverty, lack of safe drinking water, poor hygiene, malnutrition and low latrine coverage. Despite its high prevalence nowadays in Ethiopia, the direct wet mount method is used for routine laboratory diagnosis of intestinal parasites because of its feasibility as compared to other techniques even if its sensitivity is questionable. Therefore, evaluating the performance of diagnostic tests that can be used in routine patient diagnosis is essential to determine the true burden of the disease. Objective: -The objective of this study was to evaluate the performance of wet mount, Baermann, Modified Baermann and Ritchie’s methods for the diagnosis of intestinal parasites against the “Gold standard”. Methods: - A school based cross-sectional study was conducted among school children at Meshenti and Gedro elementary schools from March to June 2018. Single stool sample was processed by wet mount, Baermann, Modified Baermann and Ritchie’s methods. Data entry and analysis were done by using Statistical Package Epi-Info version 7 and Statistical Package for Social Sciences version 20 software, respectively. The sensitivity, specificity, negative predictive value and positive predictive value of diagnostic tests at 95% CI and Kappa values were calculated with respect to the “Gold standard” method (the combined result of altogether). Results: - Of the total 211 study participants, 48.8% were males and 51.2% were females. The mean age of study participants was 11.94 years. The overall prevalence of intestinal parasite infection was 60.2%. The sensitivity and negative predictive value of Wet mount, Baermann Method, Modified Baermann, and Ritchie’s methods against the “Gold standard” were: 49.6% and 56.8%, 42.5% and 53.5%, 80.3% and 77.1%, and 67.7% and 67.2%, respectively. Conclusions:-In this study, Modified Baermann outperformed the other methods followed by Ritchie’s method. Therefore, it is preferable to use Modified Baermann, in complement with Ritchie’s method as a routine laboratory diagnosis of intestinal parasitic infection in low income countries like Ethiopia.	en_US
dc.language.iso	en	en_US
dc.title	Performance evaluation of laboratory diagnostic methods for intestinal parasites among school children at rural Bahir Dar, Northwest Ethiopia	en_US
dc.type	Thesis	en_US