GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES USING ENDOD (PHYTOLACCADODECANDRA) LEAF EXTRACT AND EVALUATION OF THEIR ANTIBACTERIAL ACTIVITIES

Zelalem, Getu

dc.contributor.author	Zelalem, Getu
dc.date.accessioned	2019-04-05T05:36:03Z
dc.date.available	2019-04-05T05:36:03Z
dc.date.issued	2019-04-05
dc.identifier.uri	http://hdl.handle.net/123456789/9441
dc.description.abstract	In this study, We have used a plant mediated green synthesis of silver nanoparticles through thechemical reduction of 10 ml of 5 mM AgNO 3 solution and 1ml of 8 g Endod leaf extract areducing as well as coating (capping) agent. The move towards extracellular synthesis of silvenanoparticles using wet Endod leaf appears to be cost effective, eco-friendly to that of conventionamethods of silver nanoparticles synthesis. It was observed that the use of Endod leaf extract whicmakes a fast and convenient method for the synthesis of silver nanoparticles within a short periodof reaction time. Aqueous extract of Endod was produced and subjected to phytochemicainvestigations for determination of phenol, flavonoids, terpenoids, Quinones, Tannins, proteinsand saponin. Sunlight induced green synthesis of silver nanoparticles was performed by treatingthe aqueous extract with silver nitrate in different ratios. The formation of silver nanoparticles wavisually confirmed by the colour change of the mixture and further characterized by using UVVis absorption spectroscopy method. The UV-Visible spectrum of the colloidal solutions haabsorbance peaks observed at 424 nm. XRD is used to assess the crystallinity of synthesizednanoparticles since have high intensity or peak appear at 2θ = 38° and FTIR spectroscopconfirmed the presence of functional groups which are possibly responsible for the reduction ofsilver ions as well. The synthesized silver nanoparticles also efficient on bacterial activity whiccan be used as effective growth inhibitors against Escherichia coli (gram -Ve) and Staphylococcusaureus (gram +Ve) with an average inhibition zone from 8 mm to 9 mm respectively.Wereinhibited, as these particles have a good biocidal effect and hence effective in inhibiting bacterialgrowth. Keywords: Silver nanoparticles, Green synthesis, Endod plant, Characterizations, Antibacterial Activities	en_US
dc.language.iso	en	en_US
dc.subject	Material Science	en_US
dc.title	GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES USING ENDOD (PHYTOLACCADODECANDRA) LEAF EXTRACT AND EVALUATION OF THEIR ANTIBACTERIAL ACTIVITIES	en_US
dc.type	Thesis	en_US