ABSTRACT Antimicrobial resistance is one of our most series health problems. Infections from resistance bacteria are now common, and some pathogens have been become resistant to multiple classes of antibiotics. Transition metal based drug represents a promising approach of antibacterial agent with potential application for inhibition of bacterial infectious diseases. Consequently, in this study relatively abundant and inexpensive element Iron (III) new complexes of the ligand 2,2’-bipyrdine, [Fe(Bpy)2(H2O)2]Cl3 and mixed ligand complex of 2,2’-Bipyridine and acetamide, [Fe(Bpy)2(Ac)(H2O)]Cl3 were synthesized. The structures of the complexes were characterized using various analytical, thermal and spectroscopic techniques, particularly using UV-Vis, FT-IR, and ICP-OES. Furthermore, the synthesized metal complexes were evaluated for their in vitro antibacterial activity against Gram-positive (staphylococcus aurous, Staphylococcus pyogenes) and Gram-negative (Escherichia coli, and Klebsiella Pneumonia) bacteria by Disc Diffusion method and their antimicrobial activity were compared with the standard commercial drug Gentamycin. Both complexes showed good antimicrobial activity towards all bacteria. The results revealed that antimicrobial activity of the complexes were predominantly very effective towards strong Gram-negative bacteria Escherichia coli. Furthermore; the minimum inhibitory concentration of the complexes were determined and found that the lower the concentration of the complexes the lower the extent of inhibition. Keywords: 2, 2’-Bipyridine, Acetamide, mixed ligand complex, antibacterial activity, iron (III).