Swimming is generally considered to be a healthy leisure activity and often advised as entertainment, in treating diseases and for fitness. So, regular maintenance and water quality management is necessary to protect the users from any form of infection. The poor management of water source systems to the pools and the use of unprotected water pose a high risk of pathogen transfer to humans. Indicator organisms are often used for monitoring and regulation of recreational waters because they are common inhabitants of the intestinal tract of warm-blooded animals, found in feces at high concentration and are easier to measure in the environment than pathogens, Therefore, the aim of this study was to assess the bacteriological and physicochemical quality of water from swimming pool as well as to isolate and determine antibiotic susceptibility pattern of Staphylococcus aureus from swimming pools in Bahir Dar city. A total of 60 water samples were collected purposively from three Hotels and two public swimming pools for bacteriological analysis. Pour Plate method and Most Probable Number Methods were used for bacteriological analysis and physicochemical parameters were measured on site. The antibiotic susceptibility test for isolated S. aureus was carried out using disk diffusion method. The data that was obtained from the investigation were analyzed using SPSS version 21. From a total of 60 samples 38.4% fail to satisfy the standard limit recommended by WHO for bacteriological parameters. There was statistically significant association among bacteriological parameter (FC) and turbidity (r=0.285), pH (r=-0.282) and EC (r=-0.395) at (P<0.05) significant level. A total of 13 S. aureus isolates were isolated among them 10 (76.92%) of the isolates were resistant to each vancomycin and penicillin and all the S. aureus isolates were sensitive for gentamycin. Therefore the study reveals that there is high contamination in public pools and further study should be performed to understand the exact source of contamination. Keywords: Antibiotics, Bacteriological, Physicochemical, Swimming pool, Water quality xii