ISOLATION AND CHARACTERIZATION OF CHICKPEA (Cicer arietinum L) ROOT  ENDOPHYTIC RHIZOBACTERIA ANTAGONISTIC TO Fusarium oxysporum  (Chickpea wilt)

Sisay, Guta

dc.contributor.author	Sisay, Guta
dc.date.accessioned	2018-10-25T08:45:01Z
dc.date.available	2018-10-25T08:45:01Z
dc.date.issued	2018-10-25
dc.identifier.uri	http://hdl.handle.net/123456789/9045
dc.description.abstract	Chickpea (Cicer arietinum L) contributes 18% of the global production of grain legume and serves as an important source of dietary protein. However, decreases in cropping area and production have been challenging due to biotic and abiotic factors. Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris is the major soil-borne fungus affecting chickpeas globally. Fusarium wilt epidemics can devastate crops and cause up to 100% loss in highly infested fields under favorable conditions. Endophytic rhizobacterial isolates can provide significant biological control of Fusarium wilt of chickpea. The aim of this study was to isolate and characterize chickpea root endophytic rhizobacteria antagonistic to Fusarium oxysporum causing chickpea wilt. Samples were taken from chickpea growing areas in Adet and Andassa, Ethiopia. Endophytic rhizobacteria were isolated from the internal tissues of roots of healthy chickpea by surface sterilization method. Nine isolates were isolated of which four isolates were found to be antagonistic to Fusarium oxysporum by dual culture method on Potato Dextrose Agar (PDA). The isolate related to Bacillus (SE1B) showed maximum mean percentage inhibition of 85.7% and Klebsiella (SEAn2) showed 72.8% in dual culture plate when they were assayed separately. The efficacies of isolates in antagonizing Fusarium oxysporum increased when the effective isolates were combined. The percentage inhibition of SE1B in combination with SEAn2 was 88.6% and that of SEAn2 in combination with SE1B was 77.1%. The percentage inhibition of Serratia (SE1A) in combination with SE1B was 54.3% and that of Pseudomonus (SEAn1) in combination with SE1A was 68.5%. The mycellial growth of Fusarium oxysporum was more inhibited when effective antagonistic endophytic bacteria were combined. On the basis of morphology and biochemical tests, bacterial isolates were identified to the genus level. Three of the bacterial isolates were gram negative and Serratia (SE1A), Pseudomonas (SEAn1) and Klebsiella (SEAn2) while one isolate was Bacillus (SE1B). Isolation and screening to obtain the most promising isolates to apply in combination is more essential. Extensive research is needed to broaden the science of biocontrol and large mass application on farmland. Keywords: Antagonistic, Biocontrol, Chickpea, Endophytic rhizobacteria, Fusarium oxysporum	en_US
dc.language.iso	en	en_US
dc.subject	Biology	en_US
dc.title	ISOLATION AND CHARACTERIZATION OF CHICKPEA (Cicer arietinum L) ROOT ENDOPHYTIC RHIZOBACTERIA ANTAGONISTIC TO Fusarium oxysporum (Chickpea wilt)	en_US
dc.type	Thesis	en_US