Commercial agricultural investment has been highly recognized by the Ethiopian government to accelerate economic development and to achieve food security since 2006 in a Plan for Accelerated and Sustained Development to End Poverty (PASDEP) document. As a result of this, a large area of land has been given to both domestic and foreign investors for the production of different cash and food crops. But opposition against expropriation for commercial farming and destruction of investment sites become common incidents. Cognizant of this fact, this study assessed the PoliticalEconomy of agricultural investment in Amhara National Regional State (ANRS), particularly in Bahir Dar Zuria Woreda, taking Jovani Alphano and Tana Flora as a focus of study. Thus, a mixed research approach was employed to address research objectives. In doing so, both primary and secondary sources of data were gathered through questionnaire, interview, Focused Group Discussion (FGD), secondary sources and simple observation. A total of 160 respondents were taken to address survey question items in accordance with sample size determination formula by stratified random sampling. The data were analyzed and interpreted through statistics-by-themes and side-byside comparison in an integrative manner by way of joint display mechanisms. The study elucidated that land expropriation was carried out without genuine public consultation, ascertaining popular consent and written notification whereby the participation of peasants in land dealings was nonexistent. It had also confirmed that compensation was paid to evictees. However, the process of valuation was full of uncertainty and jumping which produced dissatisfaction and grievance on evictees. The flower farms negatively affected the livelihood and food security of peasants and it also resulted in violence and conflict, tenure insecurity, hostile and strained government-society relations, distrust and deterioration of government legitimacy. The major conclusion drawn from the finding is the expansion of commercial farming was not made vis-à-vis with empowering the displaced poor in their former status. Finally, the researcher recommended that evictees should be incorporated as part of development and rehabilitated to the extent possible by both the government and investors for the sustainability the projects.