Morphogenetic traits are physical characters of an individual and the pattern of inheritance. Morphogenetic traits of simple inheritance indicate ethnic variation and have been widely employed in population variation studies. Ten morphogenetic traits, ABO and Rh (D) blood phenotypes were investigated. The aim of this study was to find out and document data on prevalence and possible combination patterns of morphogenetic traits and ABO blood groups in three ethnic groups .Purposive sampling was used to select the ethnic groups. Data was collected randomly from 270 individuals of three ethnic groups (Awi, Amhara & Negede ethnic groups). Morphogenetic traits were observed and recorded. Blood grouping was carried out by the slide agglutination method using anti-sera (anti-A, B and D) reagents. The data were analyzed with the aid of SPSS 21.The result showed that the right handed, straight hair line shape, non-freckles, absence of cheek dimple, the ability to tongue rolling, the absence of mid digital hair, straight thumb and straight little finger were more prevalent traits in all the three study groups. Right hand clasper, and free ear lobe attachment were more prevalent in Awi and Amhara; and the invers is true in Negede. Right Hand clasper, the ability to tongue rolling and free ear lobe attachment were high in males than females while straight thumb, straight hair line shape and absence of mid digital hair were high in females. Hand clasping, handedness, cheek dimple, mid digital hair, hitchhiker’s Thumb, little finger and ear lobe attachment were significantly associated with ethnicity and hair line shape, tongue rolling and mid digital hair with gender (p<0.05) while others were not associated. Most prevalent morphogenetic trait were associated with blood group O followed by A in Awi and Amhara while blood group B in Negede. There was a significant association between hand clasping and ear lobe attachment with ABO blood groups phenotype while others were not significantly associated. Blood group O was the most prevalent (41.1%) followed by B (26.7 %), A (25.2%), while the least was AB (7.0%) in Awi and Amhara while blood group B most prevalent (54.28 %) followed by A (24.29%), O (12.86%), while the least AB (8.57%) in Negede people. The majorities (84.8%) were rhesus (D) positive and (15.2%) were rhesus (D) negative. It forms the basis for genetic counseling, genetic marker as well as make informed decisions for mothers. Further studies with large sample size at different places are more recommended to get an accurate result. Key words/Phrases: Blood group, Ethnic group, Morphogenetic traits, Rhesus factor